semithin sections  (Carl Zeiss)

Journal: Plant Direct

Article Title: Leaf Anatomy, Volatile Metabolite Profile, and Cytotoxic Activity of the Endemic Seseli tomentosum Vis.: Insights Into Species‐Specific Adaptations

doi: 10.1002/pld3.70119

Figure Lengend Snippet: (a–f) Semithin (a–d) and ultrathin (e, f) sections through the leaf of S. tomentosum , Toluidine blue (a–d). ep , epidermis; st , stomata; cu , cuticle; col , collenchyma cells; pa , palisade parenchyma cells; Kr , Kranz cells; scl , sclerenchyma cells; at , aqueous tissue; xy , xylem; ph , phloem; sd , secretory duct; sc, epithelial (secretory) cells around the duct; p , plastids (chloroplasts); od , oil droplets; arrowheads, electron‐dense secretory content within the secretory cells and phloem parenchyma cells; arrow, secretory content in the duct.

Article Snippet: Semithin sections, cut at 0.5 μm using an ultramicrotome (RMC Boeckeler, PowerTome XL), were stained with Toluidine blue O (ThermoFisher Scientific Inc., USA) to identify the area of interest.

Techniques:

Journal: Heliyon

Article Title: The choroid-sclera interface: An ultrastructural study

doi: 10.1016/j.heliyon.2022.e09408

Figure Lengend Snippet: Magnification index of figures provided.

Article Snippet: 1A , Gallus gallus , Semithin section (160x).

Techniques:

Journal: Heliyon

Article Title: The choroid-sclera interface: An ultrastructural study

doi: 10.1016/j.heliyon.2022.e09408

Figure Lengend Snippet: Chicken, 14 weeks old. A: Semithin section of the chicken sclera-choroid complex, overview: The sclera with its inner cartilaginous part and outer fibrous part and the adjacent choroid is clearly discernible, with the choroid-sclera transition zone intact. L depicts lymphatic lacunae, BV: blood vessels (containing avian nucleated erythrocytes), RPE: retinal pigment epithelium. Boxed area marks the localization of the further investigated sclera-choroid transition zone (valid for all species). B: Thin and long processes of fibroblasts form the sclera-choroid transition zone (arrowheads), connected with membrane specializations representing tight junctions (arrows). Scleral collagen fibrils (asterisks) undercut the fibroblast lamellae, reaching also into deeper layers that contain non-vascular smooth muscle cells (SMC; as identified by their intracellular striated pattern of actin-filaments). C, D: When closely investigating the fibroblast front-line lamella facing the sclera (asterisk in C, D), fibroblast (F) club-like membrane protrusions into the scleral matrix were detected (white arrowheads in C, D) as well as in the undercutting scleral collagen fibrils (black arrowheads in C, D), that were reminiscent of hook-and-loop like fasteners (with protrusions representing the hook, and the collagen matrix the loop). Fibroblasts itself were attached via tight junctions (open arrowhead). Micrograph D represents a magnification of boxed area in C. A putative difference in size of collagen fibres in chicken as seen in 1 B- to D has been interpreted as optical effect due to fibre orientation.

Article Snippet: 1A , Gallus gallus , Semithin section (160x).

Techniques: Membrane